Yeast-extract improved biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L.

Plant Cell, Tissue and Organ Culture (PCTOC), (2018), 135:347–355

Muhammad Nadeem 1, Bilal Haider Abbasi 1, 2, 3, Laurine Garros 3, 4, Samantha Drouet 3, Adnan Zahir 1, Waqar Ahmad 1 Nathalie Giglioli-Guivarc’h 2, Christophe Hano 3

1 QAU - Quaid-i-Azam University
2 BBV EA 2106 - Biomolécules et biotechnologies végétales, Université de Tours, France
3 LBLGC - Laboratoire de Biologie des Ligneux et des Grandes Cultures, Université d'Orléans, France
4 ICOA - Institut de Chimie Organique et Analytique, Université d'Orléans-CNRS, France


Lignans and neolignans are important biologically active ingredients (BAIs) biosynthesized by Linum usitatissimum. These BAIs have multi-dimensional effects against cancer, diabetes and cardio vascular diseases. In this study, yeast extract (YE) was employed as an elicitor to evaluate its effects on dynamics of biomass, BAIs and antioxidant activities in L. usitatissimum cell cultures. During preliminary experiments, flax cultures were grown on different concentrations of YE (0–1000 mg/L), and 200 mg/L YE was found to be optimum to enhance several biochemical parameters in these cell cultures. A two-fold increase in fresh (FW) and dry weight (DW) over the control was observed in cultures grown on MS medium supplemented with 200 mg/L YE. Similarly, total phenolic (TPC; 16 mg/g DW) and flavonoids content (TFC; 5.1 mg/g DW) were also positively affected by YE (200 mg/L). Stimulatory effects of YE on biosynthesis of lignans and neolignans was also noted. Thus, 200 mg/L of YE enhanced biosynthesis of secoisolariciresinol diglucoside (SDG; 3.36-fold or 10.1 mg/g DW), lariciresinol diglucoside (LDG; 1.3-fold or 11.0 mg/g DW) and dehydrodiconiferyl alcohol glucoside (DCG; 4.26-fold or 21.3 mg/g DW) in L. usitatissimum cell cultures with respect to controls. This elicitation strategy could be scaled up for production of commercially feasible levels of these precious metabolites by cell cultures of Linum.


Cell cultures
Yeast extract
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Plant Cell, Tissue and Organ Culture