Strategies to enhance cosmeceuticals in in vitro cultures of herbal plants

LE STUDIUM Multidisciplinary Journal, 2020, 4, 12-21

Bilal Haider Abbasi1,2,3, Christophe Hano3, Nathalie Giglioli-Guivarc’h2


1LE STUDIUM Institute for Advanced Studies, 45000 Orléans, France

2 Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d’Orléans, F28000 Chartres, France

3EA2106 Biomolecules et Biotechnologies Vegetales, Universite Francois-Rabelais de Tours, 37000 Tours, France

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Herbal plants accumulate large amounts of phenolics and pentacyclic triterpenes. The present research project deals with the in vitro culture induction from stem and leaf explants of several medicinal plant species of Centre-Val de Loire under various plant growth regulators (PGRs) for the production of antioxidant and anti-ageing compounds. Among all the tested PGRs, auxins and cytokinins used alone or in combination induced callogenesis in stem/leaf-derived explants. Callus culture displayed feasible total phenolic content and antioxidant activity under optimum hormonal combination. HPLC analysis revealed the presence of plectranthoic acid, oleanolic acid, betulinic acid, caffeic acid and rosmarinic acid. Complete antioxidant and anti-aging potential of extracts with very contrasting phytochemical profiles were investigated. Correlation analyses revealed rosmarinic acid as the main contributor for antioxidant activity and anti-aging hyaluronidase, advance glycation end-products inhibition and SIRT1 activation, whereas, pentacyclic triterpenoids were correlated with elastase, collagenase and tyrosinase inhibition. Altogether, these results clearly evidenced the great valorization potential of herbal plants from CVL for the production of antioxidant and anti-aging bioactive extracts for cosmetic applications.


Herbal plants
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Le STUDIUM Multidisciplinary Journal