Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans

International Journal of Molecular Sciences, 2018, 19 (4)



Sullivan Renouard (1) , Cyrielle Corbin (1) , Samantha Drouet (1) , Barbara Medvedec (1) , Joël Doussot (1, 2) , Cyril Colas (3) , Benoît Maunit (3) , Avninder Bhambra (4) , Eric Gontier (5) , Nathalie Jullian (5) , François Mesnard (5) , Michèle Boitel-Conti (5) , Bilal Haider Abbasi (6) , Randolph Arroo (4) , Christophe Hano (1)

1 Laboratoire de Biologie des Ligneux et des Grandes Cultures, LBLGC EA1207/INRA USC1328,Team Plant Lignans, Université d’Orléans, 28000 Chartres, France

2 Ecole Sciences Industrielles et Technologies de l’Information, SITI Département CASER, Le CNAM, 75141 Paris, France

3 Institut de Chimie Organique et Analytique, ICOA UMR7311, Université d’Orléans-CNRS, 45067 Orléans,France

4 Faculty of Health and Life Sciences, De Montfort University, The Gateway, Leicester LE1 9BH, UK

5 Biologie des Plantes et Interactions, BIOPI EA3900, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, F-80037 Amiens, France

6 Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan


Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.


aryltetralin lignans; deoxypodophyllotoxin; 6-Methoxy-podophyllotoxin; podophyllotoxin; hairy root; Linum flavum
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International Journal of Molecular Sciences