LE STUDIUM / Marie Skłodowska-Curie Research Fellowship
Regenerative medicine cancer research
In residence at
Dr Catherine Grillon
Effects of electro-conductive, biomaterial-based tissue scaffolds on stem cells and transdifferentiation-derived somatic cells
Hypothesis: Combination of biodegradable, conductive polymers, and/or carbon-based nanomaterials, and/or bioactive elements, electro-stimulated would cause cyclical surface changes within scaffolds, that would stimulate iPS-differentiation into other progenitors and support transdifferentiation. The project focuses on the development of novel engineered artificial electro-conductive extracellular matrix materials, and characterization of their interactions with stem cells, both under normal condition and upon electro-stimulation. The engineered extracellular matrices will be obtained from combination of biodegradable polymers like i.e. PLGA, PCL, PLA with: (I) conductive polymers i.e. PPy, PEDOT, PANI, in order to make the combined polymers electro-conductive; (II) carbon-based nanomaterials, like single-wall carbon nanotubes (SWCNT), and/or multi-wall carbon nanotubes (MWCNT); (III) combination of conductive polymers, carbon-based, and bioactive elements, in order to achieve controlled drug release. Based on the above combinations of biomaterials (I, II and III), using electrospin, and 3D-printing, new composite biomaterials will be provided by collab. partner (Dr Hudecki, Ins. Nonferrous Metals, Gliwice, Poland). The biopolymers will be tested at the Centre de Biophysique Moléculaire, (Orleans, France), for (i) biocompatibility, (ii) induction of differentiation, (iii) combined effects of electro- and biostimulation, (iv) other effects like i.e. (un)desired phenotype drifting. The results will have a broad significance for science and the society: (a) reveal events at the interface between (tissue) stem cells and electro-conductive bioscaffolds mimicking nervous system processes; new insights, into engineering of biomaterials, nanotechnology, and regenerative medicine; (b) help define future directions for the development of new-generation biomaterials; (c) fostering scientific and cultural collaboration between Malopolska and Centre Val de Loire.