From : “Sapienza” University of Rome
“Cell biology from the colloid physics perspective: a computational study of biomolecular interactions mid-way between the test-tube and the cell”
Summary of the project
The cell interior is an amazingly complex and crowded medium. Up to 40 % of its available volume is swarming with hundreds of thousands of biomolecules of all type and size, highly structured in specific compartments separated by different membranes and filled up with small organelles and a tight web of cytoskeletal structures. How proteins can possibly manage to find their unique binding partners in such an environment of mind-bogging complexity, looking more as a bustling city than a dull biological background, is still one of the most elusive puzzles in science.
The present project aims at taking an intermediate step from the test-tube to the cell stopping half-way, in the realm of colloids. With the help of the research fellow, and drawing on concepts and methods from the physics of colloids, we will build computational tools to simulate biomolecular association in complex environments. Biomolecules will be modeled as hard convex objects (HCO) or collections of freely hinged HCOs. The environment will be replenished of crowding agents of different shape (shape matters), size (exploring the effect of specific mixes) and concentration (crowding). Enthalpic interactions will be allowed for through sticky spots (local piece-wise constant potentials), thus enabling us to explore crowding effects on biomolecular association along the brim of subtle enthalpy/entropy trade-offs.