The impact of IFNγ signaling in the pulmonary damage during Plasmodium berghei ANKA infection

LE STUDIUM Multidisciplinary Journal, 2018, 2, 90-94

Norinne Lacerda-Queiroz1,2,3, Mélanie Meda, Florence Savigny, Nicolas Riteau, Valérie Quesniaux

 

1 LE STUDIUM Institute for Advanced Studies, 45000 Orléans, France

2 INEM, CNRS, UMR7355, University of Orleans, 3B rue de la Férollerie, Orleans, 45071, France

3 National Institutes of Health (NIH), Maryland, USA

Abstract

Malaria is one of the most important parasitic infection in the world. Cerebral and pulmonary complications may occur after infection and are often lethal. Immune response plays an important role in controlling malaria infection; however, excessive inflammatory response can lead to severe disease. The present work aims to decipher the cellular and molecular events associated with brain and pulmonary pathology in response to blood stage Plasmodium berghei ANKA (PbA) infection. PbA infection in C57BL/6 wild-type (WT) mice induces experimental cerebral malaria (ECM), associated with strong pro-inflammatory response, brain damage, as well as paralysis, coma early death (around day 7 p.i.). Interestingly, IFNγ receptor deficient mice (IFNγR1-/-, C57BL/6 background) are resistant to ECM and died at a later time-point, due to the hyperparasitaemia and severe anemia. Here, we addressed the impact of IFNγR1 deficiency in the development of pulmonary damage during PbA infection. At day 7 post-infection, the broncho-alveolar lavage (BAL) allowed the quantitative analysis of total cells and proteins in the broncho-alveolar space of the animals. In addition, histological analysis and Western blot were performed to compare the cerebral and pulmonary compartments. As compared to PbA-infected WT mice, the histological sections confirmed a less intense accumulation of leukocytes as well as an absence of hemorrhages in the brains of IFNγR1-/- mice. In addition, the quantification of pro-apoptotic proteins (Granzyme B and cleaved caspase-3) in olfactory bulbs showed lower levels in IFNγR1-/- mice. While IFNγR1 deficient mice were fully resistant to brain pathology, those mice were partially protected for pulmonary damage, as observed by the levels of Granzyme B and cleaved caspase-3 in the lung parenchyma, leukocyte number in the broncho-alveolar space and pulmonary edema.

Keywords

Malaria
Plasmodium
Interferon-γ
Experimental cerebral malaria
Mouse
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LE STUDIUM Multidisciplinary Journal